vitamin e  (TargetMol)

Journal: Pharmacology Research & Perspectives

Article Title: Paeonol Inhibits Cell Growth by Inducing Ferroptosis in Human Glioma Cells

doi: 10.1002/prp2.70213

Figure Lengend Snippet: Inhibition of ferroptosis decreased the cytotoxicity of PAE in U118 and U251 cells. (A, B) The phenotypes and survival of U118 and U251 cells cotreatment with ferrostatin‐1 (Fer‐1, 1 μM) orα‐tocopherol (α‐Toc, 100 μM) were examined by fluorescence microscope (scale bar: 25 μm) and a live/dead assay (scale bar: 100 μm). (C, D) The LPO and ROS level of U118 and U251 cells cotreatment with Fer‐1and α‐Toc were examined by fluorescence microscope (scale bar: 25 μm). (E) The LD level of U118 and U2511 cells cotreatment with Fer‐1and α‐Toc were examined by fluorescence microscope (scale bar: 25 μm).

Article Snippet: Paeonol (PAE), α‐Tocopherol (α‐Toc), and Ferrostatin‐1 (Fer‐1) were obtained from Med Chem Express (NJ, USA).

Techniques: Inhibition, Fluorescence, Microscopy, Live Dead Assay

Journal: Cells

Article Title: Ameliorative Effects of Vitamin E and Lutein on Hydrogen Peroxide-Triggered Oxidative Cytotoxicity via Combined Transcriptome and Metabolome Analysis

doi: 10.3390/cells14242020

Figure Lengend Snippet: Effects of vitamin E and lutein on HepG2 cell oxidative cytotoxicity. ( A ) Ameliorative effects of vitamin E and lutein at different concentrations on cell viability under 0.8 mM HP-triggered oxidative stress. ( B ) Ameliorative effects of vitamin E and lutein at different concentrations on ROS level under 0.8 mM HP-triggered oxidative stress. Different letters denote statistical significance.

Article Snippet: Vitamin E and lutein were supplied by TargetMol Chemicals Inc. (Boston, MA, USA).

Techniques:

Journal: Cells

Article Title: Ameliorative Effects of Vitamin E and Lutein on Hydrogen Peroxide-Triggered Oxidative Cytotoxicity via Combined Transcriptome and Metabolome Analysis

doi: 10.3390/cells14242020

Figure Lengend Snippet: Effects of vitamin E and lutein on antioxidant system of HepG2 cells under oxidative stress. ( A – C ) 10 μΜ vitamin E and 80 μΜ lutein improved SOD activities, CAT activities, and GSH contents under 0.8 mM HP-triggered oxidative stress. Different letters denote statistical significance.

Article Snippet: Vitamin E and lutein were supplied by TargetMol Chemicals Inc. (Boston, MA, USA).

Techniques:

Journal: Cells

Article Title: Ameliorative Effects of Vitamin E and Lutein on Hydrogen Peroxide-Triggered Oxidative Cytotoxicity via Combined Transcriptome and Metabolome Analysis

doi: 10.3390/cells14242020

Figure Lengend Snippet: Transcriptome data evaluation of vitamin E-, lutein-, or H 2 O 2 -treated HepG2 cells. ( A ) Pearson’s correlation coefficients were calculated between samples in CT, ET, HT, and LT groups. The color from blue to red indicates the coefficients from low to high. ( B ) PCA. The dots with different colors represent different groups of samples. The percentages of the explained values of PC1 and PC2 are 23.93% and 13.78%, respectively.

Article Snippet: Vitamin E and lutein were supplied by TargetMol Chemicals Inc. (Boston, MA, USA).

Techniques:

Journal: Cells

Article Title: Ameliorative Effects of Vitamin E and Lutein on Hydrogen Peroxide-Triggered Oxidative Cytotoxicity via Combined Transcriptome and Metabolome Analysis

doi: 10.3390/cells14242020

Figure Lengend Snippet: Metabolome data evaluation of vitamin E-, lutein-, or HP-treated HepG2 cells. ( A – C ) OPLS-DA score plots in comparisons of CM vs. HM, HM vs. EM, and HM vs. LM; ( D – F ) the corresponding validation plots based on 200 times permutation tests. The OPLS-DA models were valid, reliable, and stable in performance as Q2Y > 0.5 and the slope of all the blue regression lines of the Q2Y points is positive.

Article Snippet: Vitamin E and lutein were supplied by TargetMol Chemicals Inc. (Boston, MA, USA).

Techniques: Biomarker Discovery